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Young Researchers and Professionals
Project leader Radić Tomislav
Project co-leader: Nobody
Administering organization: Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21 000 Split, Croatia
Partner Institution/Company: Université catholique de Louvain - Faculté d’ingénierie biologique, agronomique et environnementale; University of Zagreb - Faculty of Agriculture
Grant type: 3A
Project title: Indigenous arbuscular mycorrhizal fungi in the grapevines from karstic soils
Project summary: The aim of the proposed project is to study arbuscular mycorrhizal fungi in the grapevines from the karstic soils of Croatia. These symbiotic fungi have exceptionally beneficial role in plants’ growth and health and hence are worldwide used as biofertilizers. In Croatia such natural soil potential has never been explored. Therefore, this project aims at establishing the collection of indigenous strains, cultivated in vivo and/or in vitro. These will serve for further scientific experiments on functioning of mycorrhizal fungi-grapevines symbiosis and selecting the effective strains for the use as inoculants in sustainable agriculture, reducing thus the role of mineral fertilizers and pesticides. The collection will be maintained in Institute for Adriatic Crops and Karst Reclamation, Split, and also in parallel in GINCO (Belgium), the world largest in vitro collection of this type of fungi. It is expected that abundance of species and strains could be found in karstic part of Croatia, since this region is known as one of the richest European parts in flora diversity and arbuscular mycorrhizal fungi usually reflect this state. Moreover, these fungi diversity declines in intensive agricultural soils, so before letting lost some indigenous strains, it is of great importance to try to isolate them from the soils where agriculture is still not notably based on mineral fertilizers and pesticides. In order to collect as more different strains as possible, samplings will be conducted in vineyards situated in different climatic conditions and where different agricultural practices are being applied. However, not only the species or strains composition are important for the functioning of their relation to grapevines, but also the seasonal changes in mycorrhizal infection of grapevine roots and their spores’ abundance, which is additional object of this project. The project activities will develop collaboration with Belgian partners who are distinguished experts in in vitro cultivation of arbuscular mycorrhizal fungi. The collaboration will also be intensified with the Faculty of Agronomy from Zagreb and lead to new collaborations within Croatia in grapevines physiology and ecology that have been ignoring mycorrhizal fungi till now. The host Institute will get a new dimension of research – soil microbiology, that has not been studied in this region before. This project will be the start of getting the applicable research results on arbuscular mycorrhizae and hopefully enable development of practical tools for Croatian grapevine farmers for transfer to sustainable agriculture.
Hrvatski sažetak: Cilj predloženog projekta je izučavanje arbuskularnih mikoriznih gljiva vinove loze u krškim tlima Hrvatske. Ove simbiotske gljive izrazito povoljno djeluju na rast i zdravlje biljaka, te se stoga diljem svijeta koriste kao biognojivo. U Hrvatskoj ovaj prirodni potencijal tla dosad nije bio istraživan. Stoga ovaj projekt namjerava uspostaviti kolekciju autohtonih sojeva, uzgojenih in vivo i/ili in vitro. Ona će služiti za daljnje znanstvene eksperimente o funkcioniranju simbioze mikorizne gljive-vinova loza i odabir učinkovitih sojeva za primjenu u održivoj poljoprivredi u obliku inokulanata, smanjujući tako upotrebu sintetskih gnojiva i pesticide. Kolekcija će se održavati u Institutu za jadranske kulture i melioraciju krša u Splitu, ali usporedno i u kolekciji GINCO (Belgija), najvećoj svjetskoj kolekciji ovog tipa gljiva. S obzirom da je krška regija Hrvatske poznata kao jedna od najbogatijih u Europi po raznolikosti biljaka, očekuje se da će se utvrditi i veliko bogatstvo vrsta i sojeva mikoriznih gljiva, jer one uglavnom odražavaju bogatstvo biljnih vrsta. Nadalje, raznolikost izučavanih gljiva opada u područjima intenzivne poljoprivrede, pa je od velike važnosti izolirati autohtone sojeve iz područja gdje mineralna gnojiva i pesticidi još uvijek nisu temelj proizvodnje. Da bi se izoliralo što više različitih sojeva, uzorkovanja će se vršiti u vinogradima u različitim klimatskim područjima i s različitim praksom uzgoja vinove loze. Za funkcioniranje odnosa ovih gljiva s vinovom lozom nije bitan samo sastav vrsta nego i sezonsko variranje stupnja njihovog koloniziranja korijenja,te brojnost spora u tlu, pa je to dodatan predmet izučavanja ovog projekta. Aktivnosti u projektu će razviti suradnju s Belgijskim partnerima, priznatim stručnjacima u in vitro uzgoju arbuskularnih mikoriznih gljiva. Također će se intenzivirati suradnja s Agronomskim fakultetom u Zagrebu u izučavanju fiziologije i ekologije vinove loze uzimajući u obzir mikorizne gljive, koje su dosad bile ignorirane. Institut na kojem će se projekt izvršavati će dobiti novu dimenziju istraživanja – mikrobiologiju tla, koja u ovoj regiji dosad nije bila izučavana. Ovim projektom će se početi dobivati primjenjivi rezultati istraživanja mikoriznih gljiva i omogućiti razvitak praktičnih “alata” za prijelaz hrvatskih vinogradara u održivi način gospodarenja tlom.
Amount requested from UKF: 259.450 HRK
Amount of matching funding: 220.000 HRK
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